Human Embryonic Stem (HUES) Cell Collection

Seventeen (17) human embryonic stem cell lines are described in Cowan, C.A. et. al, Derivation of Embryonic Stem-cell Lines from Human Blastocysts. New England Journal of Medicine. 350;13. (2004). As with all of our published reagents, it is our intention to make these cell lines as freely available to the research community as possible. In this instance, the HUES cells may be subject to patent and federal or state legislative restrictions that are beyond our control. Harvard University and the Howard Hughes Medical Institute (HHMI) have worked hard to establish a simple procedure that will enable us to provide the cells to the research community with as few encumbrances as possible.

At this time, to facilitate the transfer of HUES cells, we are not requesting any fees, though the recipient will be responsible for paying the shipping costs. Prior to sending cells, we must have a Material Transfer Agreement and Certifications executed and evidence of Embryonic Stem Cell Research Oversight Committee (ESCRO) (or equivalent) approval of your planned work with the lines by your institution.

Instructions for requesting HUES cells, the procedures for executing the necessary documents, and a lab manual containing detailed protocols can be found at the links below:
 


Human Embryonic Stem Cell Resources:

  • Requesting HUES Cells/Materials
  • Material Transfer Agreement and Certifications
  • HUES Manual/Protocol (2.35 MB PDF)
  • Contact Information


    Funding for the derivation and distribution of the HUES lines has been provided by the Howard Hughes Medical Institute, Juvenile Diabetes Research Foundation, and Harvard University.



    UNDER PRESENT POLICIES, U.S. FEDERAL FUNDS MAY NOT BE USED IN RESEARCH WITH THESE HUES CELLS
    For federally approved stem cell lines, please refer to the
    NIH STEM-CELL REGISTRY