Recent Publications
Chai Y, Chu F, Kolter R, Losick R. Bistability and biofilm formation in Bacillus subtilis. Molecular Microbiology (2007). (PDF)
Aguilar C, Vlamakis H, Losick R, Kolter R. Thinking about Bacillus subtilis as a multicellular organism. Current Opinion in Microbiology 10:638-643 (2007). (PDF)
Ferguson, C.C., Camp, A. H., Losick, R. gerT, a newly discovered germination gene under the control of the sporulation transcription factor sK in Bacillus subtilis. J Bacteriol 189:7681-7689. (2007) Aug 24. (PDF)
Chastanet A., Losick R. Engulfment during sporulation in Bacillus subtilis is governed by a multi-protein complex containing tandemly acting autolysins. Molecular Microbiology, 64:139-152 (2007). (PDF)
Ashlee M. Earl, Richard Losick, and Roberto Kolter Bacillus subtilis Genome Diversity
J. Bacteriol 189: 1163-1170 (2007). (PDF)
Ramamurthi, K.S., Clapham, K.R., Losick, R. Peptide anchoring spore coat assembly to the outer forespore membrane in Bacillus subtilis. Molecular Microbiology, 62:1547-1557 (2006). (PDF)
Bassler, B. and Losick, R. Bacterially speaking. Cell 125: 237-246 (2006). (PDF)
Dubnau, D. and Losick, R. Bistability in bacteria. Molecular Microbiology, 61:564-572 (2006). (PDF)
Ellermeier, C.D. and Losick, R. Evidence for a novel protease governing regulated intramembrane proteolysis and resistance to antimicrobial peptides in Bacillus subtilis. Genes & Development 20: (2006). (PDF)
Wang, S.T., Setlow, B., Conlon, E.M., Lyon, J.L., Imamura, D., Sato, T., Setlow, P. Losick, R., and Eichenberger, P. The forespore line of gene expression in Bacillus subtillis. J. Molec. Biol. 358:16-37 (2006). (PDF)
Ellermeier, C.D., Hobbs, Errett C., Gonzalez-Pastor, J.E., Losick R. A Three-Protein Signaling Pathway Governing Immunity to a Bacterial Cannibalism Toxin Cell 124:549-559 (2006). (PDF)
Branda, S., Chu, F., Kearns, D., Losick, R., and Kolter, R. A major protein component of the Bacilllus subtilis biofilm matrix. Molecular Microbiology 59:1229-1238 (2006) (PDF).
Silvaggi, J., Perkins, J. and Losick, R.Genes for Small, Noncoding RNAs under Sporulation Control in Bacillus subtilis. J. Bacteriol 188:532-541 (2006). (PDF)
Chu, F., Kearns, D., Branda, S, Kolter, R., and Losick, R. Targets of the master regulator of biofilm formation in Bacillus subtilis. Molecular Microbiology 59:1216-1228 (2006).
Kearns, D.B. and Losick R. Cell populations heterogeneity during growth of Bacillus subtilis Genes & Development 19:3083-3094 (2005). (PDF)
Silvaggi, J, Perkins, John B., and Losick, R. Small untranslated RNA antitioxin in Bacillus subtilis J. Bacteriol 187:6641-6650 (2005). (PDF)
Fujita, M. and Losick, R. Evidence that entry into sporulation in Bacillus subtilis is governed by a gradual increase in the level and activity of the master regulator Spo0A. Genes & Development 19:2236-2244 (2005). (PDF)
Dworkin, J., and Losick, R. Developmental commitment in a Bacterium. Cell 121:401-409 (2005). (PDF)
Carniol, K., Ben-Yehuda, S., King, N., and Losick, R. Genetic Dissection of the sporulation Protein SpoIIE and its role in asymmetric division in Bacilllus subtilis. J. Bacteriol 187:3511-3520 (2005). (PDF)
Ramamurthi, K. S., and Losick, R.M. Protein Localization: Reach out and Touch the Forespore. Current Biology. 15:R165-R167. (2005) (PDF)
Ben-Yehuda, S., Fujita, M., Liu, X., Gorbatyuk, B., Skoko, D., Yan J., Marko, J.F., Liu, J.S., Eichenberger, P., Rudner, D. and Losick, R. Defining a centromere-like Element in Bacillus subtilis by identifying the binding sites for the chromosome-anchoring protein RacA. Molec. Cell, 17: 773-782 (2005). (PDF)
Fujita, M, Gonzalez-Pastor, E, and Losick, R. High- and Low-Threshold Genes in the Spo0A regulon of Bacillus subtilis. J. Bacteriol. 187:1357-1368 (2005). (PDF)
Kearns, D., Chu, Frances, Branda, S., Kolter, R., and Losick R. A Master regulator for biofilm formation by Bacillus subtilis. Molec Microbiol 55:739-749 (2005). (PDF)
Silvaggi, J., Popham, D., Driks, A., Eichenberger, P., Losick, R. Unmasking Novel Sporulation Genes in Bacillus subtilis. J. Bacteriol 186:8089-8095 (2004). (PDF)
Eichenberger, P., Fujita, M., Jensen, S.T., Conlon, E.M., Rudner, D.Z., Wang, S.T., Ferguson, C., Haga, K., Sato, T., Liu, J.S., Losick, R. The Program of Gene Transcription for a Single Differentiating Cell Type during Sporulation in Bacillus subtilis. PLoS Biol. 2004 Sep 21;2(10):E328. (PDF) Companion website.
Branda, S, Gonzalez-Pastor, J., Dervyn, E., Ehrlich, S., Losick, R., Kolter, R. Genes Involved in Formation of Structured Multicellular Communities by Bacillus subtilis. J. Bacteriol 186:3970-3979 (2004). (PDF)
van Ooij, C., Eichenberger, P., Losick, R. Dynamic Patterns of Subcellular Protein Localization during Spore Coat Morphogenesis in Bacillus subtilis. J. Bacteriol 186:4441-4448 (2004) (PDF)
Carniol, K., Kim T., Price, C. Losick, R. Insulation of the Sigma F Regulatory System in Bacillus subtilis J. Bacteriol 186:4390-4394 (2004). (PDF)
Gehring, A., Wang, S., Kearns, D., Storer, N., Losick R. Novel Genes That Influence Development in Streptomyces coelicolor. J. Bacteriol 186:3570-3577 (2004). (PDF)
Kearns, D., Chu , F., Rudner, R. and Losick, R. Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motility. Molec. Microbiol 52:357-369 (2004). (PDF)
Carniol, K., Eichenberger, P., Losick, R. A Threshold Mechanism Governing Activation of the Developmental Regulatory Protein sf in Bacillus subtilis J. Biol. Chem. 279:14860-14870 (2004). (PDF)
Bentley, S.D., Brown, S., Murphy, L.D., Harris, D.E., Quail, M.A., Parkhill J., Barrell, B.G., McCormick, J.R., Santamaria, R.I., Losick, R., Yamasaki M., Kinashi, H., Chen, C.W., Chandra, G., Jakimowicz, D., Kieser, H. M., Kieser, T., and Chater, K.F. SCP1, a 356 023 bp linear plasmid adapted to the ecology and developmental biology of its host, Streptomyces coelicolar A3(2) Molec. Microbiol. 51:1615-1628 (2004). (PDF)
Molle, V., Fujita, M. Jensen, S., Eichenberger, P., Gonzalez-Pastor, E., Liu, J., and Losick, R. The SpoOA regulon of Bacillus subtilis. Molec Biol. 50:1683-1701 (2003). (PDF)
Ben-Yehuda, Sigal, Rudner, D., and Losick, R. Assembly of the SpoIIIE DNA Translocase Depends on Chromosome Trapping in Bacillus subtilis. Current Biology 13:2196-2200 (2003). (PDF)
Pan, Q. and Losick, R. Unique Degradation Signal for ClpCP in Bacillus subtilis. J. Bacteriol 185:5275-5278 (2003). (PDF)
Kearns, D. and Losick, R. Swarming motility in undomesticated Bacillus subtilis. Molec. Biol. 49:581-590 (2003). (PDF)
Gonzalez-Paster, E., Ho, M.S., Carniol, K., Losick, R. Evidence in Support of a Docking Model for the Release of the Transcription Factor Sigma F from the Antisigma Factor SpoIIAB in Bacillus subtilis. J. Biol. Chem. 278:20898-20905 (2003). (PDF)
Eichenberger, P., Jensen, S., Conlon, E., van Ooij, C., Silvaggi, J., Gonzalez-Pastor, J., Fujita, M., Ben-Yehuda S., Stragier, P., Lie, J. and Losick, R. The Sigma E Regulon and the Identification of Additional Sporulation Genes in Bacillus subtilis. J. Molec. Biol. 327:945-972 (2003). (PDF) Supplemental Data.
Fujita, M. and Losick, R. The master regulator for entry into sporulation in Bacillus subtilis becomes a cell-specific transcription factor after asymmetric division. Genes & Development 17:1166-1174 (2003). (PDF)
Molle, V., Nakaura, Y., Shivers, R., Yamaguchi, H., Losick, R., Fujita, Y. and Sonenshein, A. L. Additional Targets of the Bacillus subtilis Global Regulator CodY Identified by Chromatin Immunoprecipitation and Genome-Wide Transcript Analysis. J. Bacteriol 185:1911-1922 (2003). (PDF) Supplemental Data in Excel Spreadsheet van Ooij, C. and Losick, R. Subcellular Localization of Small Sporulation Protein in Bacillus subtilis. J. Bacteriol 185: 1391-1398 (2003). (PDF)
Ben-Yehuda, S., Rudner, D. and Losick, R. RacA, a Bacterial Protein That Anchors Chromosomes to the Cell Poles. Science 299:532-536 (2003). (PDF)
Shapiro, L., McAdams, Harley H. and Losick, R. Generating and Exploiting Polarity in Bacteria. Science 298:1942-1946 (2002). (PDF)
Dworkin, J. and Losick, R. Does RNA polymerase help drive chromosome segregation in bacteria? Proc. Natl. Acad. Sci. USA 99:14089-94 (2002). (PDF)
Gueiros-Filho, Frederico J. and Losick, R. A widely conserved bacterial cell division protein that promotes assembly of the tubulin-like protein FtsZ. Genes & Dev. 16:2544-2556 (2002). (PDF)
Britton, Robert A., Eichenberger, P., Gonzalez-Pastor, J., Fawcett, P., Monson, R., Losick, R. and Grossman, A. Genome-Wide Analysis of the Stationary-Phase Sigma Factor (Sigma-H) Regulon of Bacillus subtilis. J. Bacteriol. 184:4881-4890 (2002). (PDF) Supplemental Data
Rudner, D., Pan, Q. and Losick, R. Evidence that subcellular localization of a bacterial membrance protein is achieved by diffusion and capture. Proc. Natl. Acad. Sci 99:8701-8706 (2002). (PDF)
Kemp, J., Driks, A. and Losick, R. FtsA Mutants of Bacillus subtilis Impaired in Sporulation. J. Bacteriol. 184:3856-3863 (2002). (PDF)
Nohturfft, A. and Losick, R. Fats, Flies, and Palmitate. Science 296:857-858 (2002). (PDF)
Rudner, D. Z. and Losick, R. A sporulation membrane protein tethers the pro-Sigma K processing enzyme to its inhibitor and dictates its subcellular localization. Genes & Dev. 16: 1007-1018 (2002). (PDF)
Piggot, P. J. and Losick, R. Sporulation Genes and Intercompartmental Regulation. In Bacillus subtilis and Its Closet Relatives: from Genes to Cells, eds. A. L. Sonenshein, J. Hoch and R. Losick, ASM Press, Washington, D.C., (2002).
Ben-Yehuda, S. and Losick, R. Asymmetric Cell Division in B. subtilis Involves a Spiral-like Intermediate of the Cytokinetic Protein FtsZ. Cell 109:257-266 (2002). (PDF)
Fujita, M. and Losick, R. An investigation into the compartmentalization of the sporulation transcription factor Sigma E in Bacillus subtilis. Molec. Microbiol. 43:27-38 (2002). (PDF)
Rudner, D. Z. and Losick, R. Morphological Coupling in Development: Lessons from Prokaryotes. Developmental Cell 1:733-742 (2001). (PDF)
Eichenberger, P., Fawcett, P. and Losick, R. A three-protein inhibitor of polar septation during sporulation in Bacillus subtilis. Molec. Microbiol. 42:1147-1162 (2001). (PDF)
Dworkin, J. and Losick, R. Differential Gene Expression Governed by Chromosomal Spatial Asymmetry. Cell 107:339-346 (2001). (PDF)
Pan, Q., Garsin, D. A. and Losick, R. Self-Reinforcing Activation of a Cell-Specific Transcription Factor by Proteolysis of an Anti-Sigma Factor in B. subtilis. Molecular Cell 8:873-883 (2001). (PDF)
Branda, S. S., Gonzalez-Pastor, J. E., Ben-Yehuda, S., Losick, R. and Kolter, R. Fruiting body formation by Bacillus subtilis. Proc. Natl. Acad. Sci. USA 98:11621-11626 (2001). (PDF)
Gehring, A. M.,Yoo, N.J., Losick, R. RNA Polymerase Sigma Factor That Blocks Morphological Differentiation by Streptomyces coelicolar. J. Bacteriol, 183:5991-5996 (2001). (PDF)
Losick, R. and Sonenshein, A. Turning Gene Regulation on Its Head. Science, 293:2018-2019 (2001). (PDF)
Dworkin, J. and Losick, R. Linking nutritional status to gene activation and development. Genes & Development 15:1051-1054 (2001). (PDF)
Yang, M. and Losick, R. Cytological Evidence for Association of the Ends of the Linear Chromosome in Streptomyces coelicolor. J. Bacteriol. 183:5180-5186 (2001). (PDF)
Grauman, P. and Losick R. Coupling of asymmetric division to polar placement of replication origin regions in Bacillus subtilis J. Bacteriol. 183:4052-4060 (2001). (PDF)
Gehring, A., Nodwell, J., Beverley, S. and Losick, R. Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation. Proc. Nat. Acad. Sci. USA 97:9642-9647 (2000). (PDF)
Fawcett, P., Eichenberger, P., Losick, R. and Youngman, P. The transcriptional profile of early to middle sporulation in Bacillus subtilis. Proc. Nat. Acad. Sci. USA 97:8063-8068 (2000). (PDF)
Supplemental Data.
Shapiro, L. and Losick, R. Dynamic Spatial Regulation in the Bacterial Cell. Cell 100:89-98 (2000). (PDF)
